Table 2.
Gene expression of pro- and anti-inflammatory cytokines in immunocompetent and immunocompromised neonates. Gene expression of cytokines and chemokine in measles virus-infected CD46+, CD46+/IFNγ-KO and CD46+/RAG2-KO neonates. Brain tissue was collected from uninfected and infected neonates and RNA was extracted at 7 dpi. qRT-PCR array analysis was performed using the RT2 Profiler™ PCR Array. Changes in the gene expression that were more than two-fold relative to the uninfected controls are shown (p < 0.05 by student t test). All data were normalized against levels of housekeeping genes within the same sample.
| Gene | Description | CD46+ neonate |
CD46+/IFNγ-KO neonate |
CD46+/RAG2-KO neonate |
|||
|---|---|---|---|---|---|---|---|
| Fold change | p-Value | Fold change | p-Value | Fold change | p-Value | ||
| Cxcl10 | Chemokine (C-X-C motif) ligand 10 | 84.62 | 0.011 | 291.38 | 0.001 | 771.13 | 0.023 |
| IL1rn | Interleukin 1 receptor antagonist | 17.84 | 0.016 | 68.96 | 0.003 | 214.9 | 0.0008 |
| Ccl5 | Chemokine (C-C motif) ligand 5 | 15.18 | 0.007 | 33.49 | 0.013 | 132.97 | 0.013 |
| IFNγ | Interferon gamma | 11.08 | 0.027 | – | – | 15.02 | 0.023 |
| Ccl4 | Chemokine (C-C motif) ligand 4 | 10.54 | 0.012 | 32.55 | 0.007 | 153.19 | 0.0001 |
| Ccl12 | Chemokine (C-C motif) ligand 12 | 5.69 | 0.032 | – | – | 272.95 | 0.001 |
| Cxcl13 | Chemokine (C-X-C motif) ligand 13 | 3.89 | 0.029 | 14.11 | 0.016 | 41.24 | 0.006 |
| Ccl3 | Chemokine (C-C motif) ligand 3 | 3.73 | 0.021 | 8.35 | 0.003 | 31.05 | 0.0001 |
| IL1β | Interleukin 1 beta | 3.27 | 0.027 | 5.9 | 0.003 | 9.85 | 0.005 |
| Ccl2 | Chemokine (C-C motif) ligand 2 | 15.33 | 0.042 | 35.89 | 0.018 | 493.99 | 0.001 |
| Ccl7 | Chemokine (C-C motif) ligand 7 | 9.31 | 0.017 | 16.46 | 0.024 | 164.18 | 0.024 |
| Tnf | Tumor necrosis factor | 8.36 | 0.016 | 17.9 | 0.007 | 72.05 | 0.009 |
| IL12b | Interleukin 12B | 7.87 | 0.02 | 15.28 | 0.008 | 199.93 | 0.03 |
| Osm | Oncostatin M | 4.44 | 0.009 | 5.58 | 0.024 | 11.29 | 0.023 |
| Xcl1 | Chemokine (C motif) ligand 1 | 3.84 | 0.03 | 13.98 | 0.009 | 21.72 | 0.047 |
| IL27 | Interleukin 27 | 3.4 | 0.031 | 6.49 | 0.001 | 14.55 | 0.005 |
| Cxcl11 | Chemokine (C-X-C motif) ligand 11 | 2.58 | 0.025 | 5.23 | 0.007 | 17 | 0.018 |
| IL10 | Interleukin 10 | 2.36 | 0.03 | – | – | – | – |
| Cxcl9 | Chemokine (C-X-C motif) ligand 9 | 9.08 | 0.004 | 71.63 | 0.008 | ||
| IL6 | Interleukin 6 | 4.77 | 0.009 | 16.85 | 0.004 | ||
| B2m | Beta 2 microglobulin | 4.49 | 0.002 | 11.86 | 0.024 | ||
| IL1α | Interleukin 1 alpha | 3.56 | 0.0003 | 3.66 | 0.021 | ||
| CXCL1 | Chemokine (C_X_C) ligand 1 | 2.26 | 0.036 | – | – | ||
| Ccl11 | Chemokine (C-C motif) ligand 11 | 11.17 | 0.012 | ||||
| Cxcl16 | Chemokine (C-X-C motif) ligand 16 | 7.23 | 0.0001 | ||||
| CSF1 | Colony stimulating factor 1 | 6.92 | 0.0001 | ||||
| IL15 | Interleukin 15 | 6.12 | 0.0009 | ||||
| Ccl22 | Chemokine (C-C motif) ligand 22 | 6.18 | 0.012 | ||||
| Ccl19 | Chemokine (C-C motif) ligand 19 | 5.75 | 0.0001 | ||||
| Tnfsf10 | TNF (ligand) superfamily, member 10 | 5.55 | 0.0002 | ||||
| IL5 | Interleukin 5 | 3.68 | 0.03 | ||||
| Ccl17 | Chemokine (C-C motif) ligand 17 | 3.56 | 0.04 | ||||
| CNTF | Ciliary neurotrophic factor | 3.31 | 0.008 | ||||
| IL3 | Interleukin 3 | 3.09 | 0.04 | ||||
| Tnfsf13b | TNF (ligand) superfamily, member 13b | 2.43 | 0.045 | ||||
| IL7 | Interleukin 7 | 2.34 | 0.046 | ||||
| Bmp7 | Bone morphogenetic protein 7 | 3.9 | 0.02 | ||||
| Bmp6 | Bone morphogenetic protein 6 | 3.4 | 0.004 | ||||
| Bmp4 | Bone morphogenetic protein 4 | 3 | 0.024 | ||||
| Bmp2 | Bone morphogenetic protein 2 | 2.85 | 0.036 | ||||
| Mstn | Myostatin | 2.52 | 0.029 | ||||