Table 4.

Potencies and efficacies of the Dyn B amide alanine analogs at KOR in the GTPγS assay

Peptide	EC50 (nM ± SEM)a,b
N-Terminal alanine substituted analogs
1 [Ala1]	2650 ± 890
2 [Ala4]	1180 ± 330
C-Terminal alanine substituted analogs
3 [Ala5]	109 ± 34
4 [Ala6]	116 ± 21
5 [Ala7]	802 ± 290
6 [Ala8]	20.2 ± 10.8
7 [Ala9]	14.8 ± 2.6
8 [Ala10]	6.36 ± 1.70
9 [Ala11]	8.79 ± 2.20
Dyn B NH2	9.14 ± 1.96

^aValues are for n ≥ 2.

^bAll analogs exhibited ≥90% efficacy as compared to Dyn A(1-13)NH₂ (efficacy = 100%) except for Ala¹⁰ (86 ± 3% efficacy).