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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 Dec 15;142(1):219–234. doi: 10.1016/j.jaci.2017.11.023

Figure 6. R-loop load modulates the steady-state mRNA expression patterns in human Th1-cells.

Figure 6

(A) Heat maps reporting on the RT-qPCR-based mRNA expression profiles of NF-κB response genes for WAS Th1-line, untransfected (control), or transfected with either RNaseH1 or wt-WASp using the 96-well plate format from Qiagen. The up/down gene modulation shown is in the context of transitioning from Th0-unstimulated to Th1-skewed state, in vitro. Red, gene up-regulation, Green, gene down-regulation, black, unchanged. Also see Supplementary file 4 for actual values. (B) Bar graphs display the actual values (fold-change) of the indicated 4 genes derived from the mRNA expression array (heat-maps) shown in panel B. IFNG is at C-03 and STAT1 at E-03 in the heat-map layout, and both become upregulated, whereas, CSF2 (GM-CSF) is at B05, TNFAIP2 is at G-11, both pro-inflammatory cytokine genes become downregulated in WAS Th1-cells upon expressing RNaseH1 or wt-WASp. (C) RT-qPCR. Fold change in the mRNA expression of 3 indicated Th1-genes in WAS Th-line (Th1-skewed) relative to the same WAS Th-line, untransfected (control) or transfected with RNaseH1. n=3, mean +SEM.