Schematic representation of cell cycle progression during appressorium development in the rice blast fungus M. oryzae. The fungus three-celled conidium adheres to the plant hydrophobic cuticle and germinates. Each cell contains one nucleus. One of the nuclei migrates to the germ tube and goes through a single mitotic event, in which two daughter nuclei are generated in a closed mitosis. On daughter nucleus migrates into the immature appressorium and a septum is formed, while the other nucleus migrates back to the conidium, which then goes through an autophagic cell death process. Appressorium remains mitotically active after maturation. Brown circles: nucleus, dashlines: conidium degradation, HI: invasive hyphae.