TABLE 2.

Dynamic range, linearity, accuracy, and precision of eicosanoids in human serum

Analytes ID	Dynamic range (ng/mL)	LLOQ (0.2 ng/mL)		LQC (1 ng/mL)		MQC (30 ng/mL)		HQC (100 ng/mL)		ULOQ (500 ng/mL)
		Accuracy	RSD (%)	Accuracy	RSD (%)	Accuracy	RSD (%)	Accuracy	RSD (%)	Accuracy	RDS. (%)
1	0.2–500	111	9	95	15	90	1	98	4	112	4
4	0.2–500	101	10	98	4	91	3	92	4	90	3
6	0.2–500	99	14	101	10	94	3	94	4	94	4
7	0.2–500	109	12	94	6	101	2	104	3	113	2
14	0.2–500	104	10	88	9	92	6	94	5	114	3
17	0.2–500	110	8	105	11	97	5	99	6	115	5
18	0.2–500	110	8	87	3	85	1	87	9	100	2
20	0.2–500	99	6	103	9	92	3	97	4	97	1
21	0.2–500	105	9	101	13	99	4	109	4	115	1
22	0.2–500	103	13	88	7	91	6	103	5	116	1
24	0.2–500	94	11	109	3	100	1	99	1	106	1
28	0.2–500	106	12	92	13	91	4	95	2	103	4
29	0.2–500	104	12	100	10	103	5	108	3	108	3
30	0.2–500	105	8	101	11	96	2	101	2	108	2
31	0.2–500	90	11	90	10	93	5	89	6	85	1
32	0.2–500	103	7	86	5	86	3	86	5	115	3
33	0.2–500	103	11	97	11	96	3	97	8	93	3
34	0.2–500	107	14	79	6	88	3	85	5	90	3
35	0.2–500	93	12	92	9	100	3	103	11	96	10
36	0.2–500	110	9	96	14	88	3	90	2	114	3
37	0.2–500	105	15	90	5	94	4	99	3	98	1
38	0.2–500	105	13	94	8	90	3	97	1	104	3
39	0.2–500	96	14	103	5	95	4	95	6	107	2
44	0.2–500	103	5	102	7	102	4	100	2	112	2
45	0.2–500	96	13	105	10	90	1	94	3	113	2
		LLOQ (1 ng/mL)		LQC (3 ng/mL)		MQC (30 ng/mL)		HQC (100 ng/mL)		LOW (500 ng/mL)
19	1–500	104	13	10.2	10	90	2	93	4	85	1
23	1–500	105	11	6.0	6	97	8	96	8	107	3
40	1–500	102	7	2.4	2	89	4	100	4	89	3
		LLOQ (3 ng/mL)		LQC (10 ng/mL)		MQC (100 ng/mL)		HQC (350 ng/mL)		ULOQ (500 ng/mL)
9	3–500	115	4	4.7	5	115	4	103	3	100	5
10	3–500	110	10	6.5	6	115	3	105	7	99	6
11	3–500	112	6	5.4	5	107	3	103	7	102	5
12	3–500	97	15	6.2	6	114	7	106	9	99	5
13	3–500	110	12	7.4	7	105	5	98	5	94	4
43	3–500	95	12	11.6	12	105	9	110	11	106	12