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. Author manuscript; available in PMC: 2019 Jun 14.
Published in final edited form as: Cell. 2018 May 10;173(7):1678–1691.e16. doi: 10.1016/j.cell.2018.03.066

Fig. 3. SIM images of late prophase CO-designated recombination sites.

Fig. 3

(A) Left: Representative late pachytene nuclei (top) and enlargements of portions of this field (below). Right: Individually-cropped recombination sites. Examples displayed show CO-designated sites at chromosome segments imaged in frontal aspect, illustrating two populations of BLM (oriented in parallel with the chromosome axes), two populations of MSH-5 (oriented perpendicular to the axes) and a single population of COSA-1 at the center. Scale bars: 1 μm (left) and 500 nm (right). (B) Top: diplotene nuclei; most BLM signals are no longer reliably resolved as doublets; arrowheads highlight rare examples of BLM doublets. Scale bar: 1 μm. Bottom: Individually-cropped diplotene recombination sites. Scale bar: 500 nm. (C) Diakinesis nucleus. Red arrowheads indicate chiasmata, temporary connections between desynapsed homologs that reflect the formation of COs; BLM and MSH-5 are no longer detected. Scale bar: 1 μm.