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. 2018 Jun 15;8:9166. doi: 10.1038/s41598-018-27525-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hypoxic-ischemic insult disrupts the vascular development in the murine neonatal retina. Representative photomicrographs were taken from the middle to the periphery of cryosectioned P12 control and P9D3 HIE retinas. Blood vessels were labeled with Collagen IV and all retinal nuclei were labeled with DAPI. Collagen IV staining (red) demonstrate an approximate complete coverage of the deep vascular layer (arrow) to the periphery of the P12 control retinas (arrow head) but not of the P9D3 HIE retinas. Eyes from three mice per group were examined. Scale bar, 20 µm.