Figure 2.

Hypoxic-ischemic insult disrupts the vascular development in the murine neonatal retina. Representative photomicrographs from the middle to the periphery of the retinas were taken from P9 control, P12 control, P9D3 HIE, P100 control, and P9D90 HIE mice. Blood vessels were labeled with Collagen IV or Griffonia simplicifolia isolectin B4. Vessels in different retinal layers were given pseudo-colors (Red, deep vascular layer; Green, intermediate vascular layer; blue, superficial layer). Because of retinal thinning, the superficial vascular layer at the edge of periphery is at the same plane of the lagging deep vascular layer; Both layers are separated by the white solid line. Retinas from at least five mice per group were examined. Scale bar, 50 µm.