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. 2018 Jun 15;8:9166. doi: 10.1038/s41598-018-27525-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hypoxic-ischemic insult occasionally results in the formation of neovascularization tufts. Representative photomicrographs were taken from P12 control, P9D3 HIE, and P9D30 HIE retinas (A), P7H5N1, P7H5N3, and P7H5N27 retinas and (B), P9H5N1, P9H5N3, and P9H5N25 retinas (C). Blood vessels were labeled with Griffonia simplicifolia isolectin B4 or collagen IV. Neovascular tufts are marked by arrows. Arrowheads indicate unique abnormal vascular structures that do not protrude toward the vitreous. Retinas from at least four mice per time-point were examined. Scale bar, 200 µm.