Figure 5.

Astrocyte distribution and density were altered in the retinas of HI mice. Representative photomicrographs were taken from P12 and P100 control and P9D3 and P9D90 HIE mice. Flat-mount retinas were labeled for isolectin B4 and GFAP, an intermediate filament protein expressed in astrocytes an in activated Müller cells. Two separate maximum projection images were generated per representative sample at the superficial and deep vascular layers. Retinas from control animals showed typical astrocyte distribution and density (stellate-shaped astrocytes which makes discrete contacts on blood vessels)³⁵. On the other hand, the majority of HI retinas showed altered astrocytes distribution and density and activation of Müller cells. Arrows represent the activated Müller cells. Retinas from at least five mice per group were examined. Scale bar, 200 µm.