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. 2018 Jun 15;8:9166. doi: 10.1038/s41598-018-27525-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hypoxic-ischemic insult causes degeneration of murine neonatal neuroretina. Eyes were processed and paraffin-sectioned through the optic disc then hematoxylin and eosin stained. Representative photomicrographs were taken for the whole eye section and from the center, the middle and the periphery of the eye. Postnatal day 9 (P9) control eye represents the eye structure before the hypoxia-ischemia was initiated; P12 control eye; P9D3 HIE represent P12 mouse (3 days post-HI); P100 control eye; P9D90 represent P99 mouse (90 days post-HI). Abbreviations: GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; and ONL: outer nuclear layer. Images were captured on Aperio slide scanner. Eyes from three mice per group were examined. Scale bar, 200 µm.