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. 2018 Jun 15;8:9204. doi: 10.1038/s41598-018-27394-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cells expressing BRAF^V600E and KRAS^G12V are resistant to MCT1 inhibition. (A) Sensitivity analysis of lactic acid concentration depending on the MCT enzyme concentration. The MCT enzyme concentration varies here from 25% to 200% of the fitted value. The model shows how changing the MCT concentration affects the lactic acid accumulation for each cell line model. Low MCT enzyme concentrations result in increasingly large lactic acid concentrations. (B,C) CaCO2-control, CaCO2-BRAF^V600E, CaCO2-KRAS^G12V, HT29 and SW480 cells grown in physiological (1.0 g/L) and intermediate (2.5 g/L) glucose concentrations were treated with 0.1 µM MCT1 inhibitor SR13800 (+), 10 µM AZD3965 (+) or DMSO (−) for 24 h. Viable cells compared to DMSO are depicted. Shown are standard deviation of (in minimum) n = 3 replicates. p < 0.05 was indicated with asterisk (unpaired two-tailed t Test). glc: glucose.