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. 2018 Jun 15;8:9221. doi: 10.1038/s41598-018-27626-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of H₂ on LPS-induced neuronal loss. (A) Representative images of Nissl staining; amygdala and cortex. Scale bar = 100 μm. (B) The quantitation of the number of Nissl-positive cells was performed as described in the Materials and Methods. The result is expressed as the mean ± standard error of the mean (n = 12 in each group). Data are analyzed by one-way ANOVA with Tukey’s honest significant difference test. *p < 0.05, **p < 0.01, ***p < 0.001. LPS, lipopolysaccharide.