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. 2018 Jun 15;8:9196. doi: 10.1038/s41598-018-27575-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impaired uSA remodeling of BPA-treated mice at gd10. uSA thickness (A) and wall-to-lumen ratios (B) from C57BL/6J control (n = 7) and BPA-treated C57BL/6J mice (n = 5) were calculated by measuring wall and lumen diameters of 3–8 uSAs per mouse. Results are presented as means ± S.E.M. and analyzed by using Mann-Whitney-U-test (**P < 0.01). (C) Representative images of uSAs from H/E-stained implantations of C57BL/6J (i) and BPA-treated C57BL/6J (ii) are shown (scale bars = 100 µm). (D) Representative smooth muscle actin immunfluorescence staining images of SAs from implantations of BL/6J (i-iii) and BPA-treated BL/6J (iv-vi) are shown (scale bars = 100 µm). uSA, uterine spiral artery; gd, gestation day.