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. 2018 Jun 15;8:9163. doi: 10.1038/s41598-018-27427-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in liver mass and development of fibrosis. The graphs show the liver-to-body weight ratio (A), and the area of fibrosis (%) evaluated in liver tissue sections stained with (B) Sirius Red or (C) Masson’s Trichrome in rats receiving oral gavage with water (Control group), CCl4 once a week (CCl4-1xWk group) or CCl4 twice a week (CCl4-2xWk) for 12 weeks following the corresponding protocols. Horizontal lines represent the median. *p < 0.05, **p < 0.01, ****p < 0.0001 (ANOVA with Tukey’s post-hoc tests).