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. 2018 Jun 15;8:9202. doi: 10.1038/s41598-018-27379-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Investigating Dcaf17 mRNA levels by real time PCR. (A) Relative expression of Dcaf17 in different tissues of adult mouse. Relative expression values of Dcaf17 mRNA were normalized to 18S rRNA expression and the level of Dcaf17 mRNA in the brain was arbitrarily set at 1. The Dcaf17 is highly expressed in testis, while other tissues show low level of expression. (B) Relative expression of Dcaf17 in testis at different ages. Relative expression values of Dcaf17 mRNA were normalized to 18S rRNA expression and the level of Dcaf17 mRNA in the 5 days postpartum (dpp) testis was arbitrarily set at 1. Expression of Dcaf17 in the testis increased with the age till the age of 32 dpp and then after it remained constant. Asterisks indicate statistical significance (p < 0.0001 for A and p < 0.0036 for B). Error bars represent SEM. Three to five animals were used to investigate the transcript levels of Dcaf17. Experiments were performed in triplicates. Data were analyzed on Graphpad prism 5 software using one-way ANOVA technique followed by post hoc Bonferroni’s (A) or Tukey’s (B) multiple comparison test. ns - not significant.