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. 2018 Jun 15;8:9202. doi: 10.1038/s41598-018-27379-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence analysis of meiotic spermatocytes of 8 weeks old WT and Dcaf17^−/− testes. Immunostaining of the synaptonemal complex with SYCP1 (green) and SYCP3 (red) antibodies in pachytene spermatocytes of WT and Dcaf17 mutant mice (Panel A). Immunofluorescnece staining of γ-H2AX (red) and the lateral element of the synaptonemal complex, SYCP3 (Green) in pachytene spermatocytes of WT and Dcaf17^−/− mice (Panel B). Spermatocytes with synaptic X and Y chromosomes (green) around which γ-H2AX (red) was normally distributed in WT and Dcaf17^−/− strains. Nucleus is counter stained with DAPI (blue). Magnification 1000X. Scale bar: 10 µm.