Table 2.

List of primers used in this study.

Primer Name	Primer Sequence (5′-3′)	Purpose
Sp6	GAGTGCACCATATGGACATATTGTC	Sequencing targeting vector
T7	TAATGCAGGTTAACCTGGCTTATCG	Sequencing targeting vector
N1	TGCGAGGCCAGAGGCCACTTGTGTAG	Sequencing targeting vector
N2	TTCCTCGTGCTTTACGGTATCG	Sequencing targeting vector
CARF3	TGCTCTAGTTGAAGACTTGAGG	Sequencing targeting vector
A2	ACA CGT TCA TGT ATG AGC CCA G	ESC screening
A1	ACACTCTGAGGTAGTTTATAGTCG	ESC screening
UNI	AGCGCATCGCCTTCTATCGCCTTC	ESC screening
LOX1	TTGCATACTCACATCCATTTGG	F1 and floxed mice genotyping/LoxP site retention
SDL2	TGCTCTAGTTGAAGACTTGAGG	F1 and floxed mice genotyping/LoxP site retention
LAN1	CCAGAGGCCACTTGTGTAGC	F1 genotyping/LoxP site retention
WSS-F	TTGCTCCTCTTTCCCTTCTG	Dcaf17 KO mice genotyping
WSS-R	CAAATTGCATACTCACATCCATT	Dcaf17 KO mice genotyping
Dcaf17-3F	GTTGCATCAGAGCCAAGAAA	RT-PCR
Dcaf17-5R	TTGTTCTGAGCCGACTTCAC	RT-PCR
Dcaf17-F	GTTGTACCTCGCAGTGTTCC	qRT-PCR
Dcaf17-R	GATGGCTTGGAAGCTGTAGA	qRT-PCR
18 s rRNA-F	CTCTTTCGAGGCCCTGTAAT	qRT-PCR
18 s rRNA-R	CTCCCAAGATCCAACTACGA	qRT-PCR