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. 2018 Jun 15;8:9215. doi: 10.1038/s41598-018-27533-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Analytical sensitivity of three RT-qPCR assays for bovine astrovirus CH13/NeuroS1 (BoAstV-CH13/NeuroS1). In vitro transcribed genomic RNA was serially diluted tenfold and tested in the CH13-A, CH13-B and the CH13-C assays. Positive results were obtained for ≥1.34 × 10² RNA copies (cut-off ct ≤35). Calibration curves were generated by linear regression and the resulting linear regression coefficients (R²) were ≥ 0.99. (B) Conventional pan-astrovirus RT-PCR using the same dilution of the BoAstV-CH13/NeuroS1 RNA transcript. Molecular masses of the DNA marker are indicated in bp on the right. The image has been cropped and rotated using the CorelDRAW X6 software. The full-length gel is presented in Fig. S2.