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. 2018 Jun 15;8:9215. doi: 10.1038/s41598-018-27533-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Accuracy of bovine astrovirus CH13/NeuroS1 (BoAstV-CH13/NeuroS1) RT-qPCR assays. The diagnostic sensitivity was determined by testing brain tissue samples with non-suppurative encephalitis and a BoAstV-CH13/NeuroS1 positive status (encephalitis+/astrovirus+) (n = 20), and the diagnostic specificity by testing samples without histopathological brain lesions and with a BoAstV-CH13/NeuroS1 negative status (encephalitis−/astrovirus−) (n = 20). Finally, the analytical specificity was assessed on samples with a non-suppurative lesions pattern, in which no virus was detected (n = 20), and on samples with encephalitis, in which viruses other than BoAstV-CH13/NeuroS1 were identified (n = 17). The positive/negative cut-off at cycle threshold (ct) 35 is indicated by a dashed line. Individual astrovirus positive samples are coded by colors.