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. 2018 Jun 15;8:9215. doi: 10.1038/s41598-018-27533-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Precision of bovine astrovirus CH13/NeuroS1 (BoAstV-CH13/NeuroS1) RT-qPCR assays. (A) Intra-test repeatability of the three assays (CH13-A, CH13-B and CH13-C) was assessed by testing two replicates of 20 different BoAstV- CH13/NeuroS1 tissue RNA extracts in the same test run. (B) Inter-test repeatability was determined by testing BoAstV-CH13/NeuroS1 positive (n = 2; 23985 and 26875) and negative tissue homogenates (n = 2) in 20 independent test runs with separate RNA extraction. Negative tissue homogenates tested consistently negative (data not shown). For positive tissue extracts, individual ct values (dots) are presented together with average scores and standard deviations (error bars). The positive/negative cut-off at cycle threshold (ct) 35 is indicated by a dashed line.