Table 1.
Details on bovine astrovirus-CH13/NeuroS1 positive animals and brain tissues used in the present study.
| Animal ID | Age [years] | Morphological diagnosis | Virus characterisation methodsa | References | Tissues used in this study |
|---|---|---|---|---|---|
| 23985 | 2.5 | Severe non-suppurative meningoencephalitis | RT-PCR, ISH, NGS, IHC | 5,18,21 | cerebral cortex |
| 26730 | 4 | Viral meningoencephalitis | ISH, NGS, IHC | 18,21 | cerebral cortex, cerebellum |
| 26875 | 1.5 | Viral meningoencephalitis | RT-PCR, ISH, NGS, IHC | 5,21 | brainstem |
| 27020 | 3 | Viral meningoencephalitis | ISH, IHC | 18 | cerebral cortex, cerebellum |
| 36716, | 2 | Non-suppurative meningo-encephalopmyelitis | ISH, NGS, IHC | 18,21 | cerebral cortex, cerebellum, brainstem |
| 37514 | 2.5 | Moderate non-suppurative meningo-encephalopmyelitis | RT-PCR, ISH, IHC | 5,14 | cerebral cortex |
| 42268 | 4 | Moderate non-suppurative meningo-encephalopmyelitis | ISH, IHC | 14 | cerebral cortex, cerebellum, brainstem |
| 42799 | 6.5 | Severe non-suppurative polioencephalomyelitis | RT-PCR, ISH, NGS, IHC | 5,8,14,21 | brainstem |
| 43660 | 4.5 | Moderate non-suppurative meningo-encephalitis | ISH, NGS | 14,21 | brainstem |
| 45664 | 1.5 | Severe meningoencephalomyelitis | RT-PCR, ISH, IHC | 5,18 | cerebral cortex, cerebellum |
| 49852 | 4.5 | Severe non-suppurative encephalitis | RT-PCR, ISH, IHC | 14,24 | brainstem |
| 50773 | 3.5 | Severe non-suppurative poliomeningoencephalitis | RT-PCR, ISH, IHC | 18,21 | cerebral cortex, cerebellum |
aRT-PCR, reverse-transcription polymerase chain reaction; ISH, in situ hybridization; IHC, immunohistochemistry; NGS, next-generation sequencing.