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. 2018 Jun 15;8:9168. doi: 10.1038/s41598-018-27601-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of ROBO1/2 interference on biomarker gene expression and GC proliferation. In the GCs transfected with or without the reconstructed pYr-adshuttle-4-SLIT2 plasmids, the cells were co-transfected with specific siRNAs targeting the ROBO1 and/or ROBO2 genes. GC proliferation was examined by an EdU incorporation assay (original magnification × 20). (A) The effect of the ROBO1 interference on granulosa cell proliferation. (B) The effect of the ROBO2 interference on granulosa cell proliferation. (C) The effect of the ROBO1 and ROBO2 co-interference on granulosa cell proliferation. (D) The effect of the ROBO1 and ROBO2 co-interference on the mRNA expression levels of the biomarker genes FSHR, GDF9, STAR and CYP11A1. For each group, the different superscript above the bar indicates that the difference was significant (P < 0.01).