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. 2018 Jun 15;8:9176. doi: 10.1038/s41598-018-27458-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of fat-free diet on ghrelin production in mice. After 3 weeks of feeding with the control or fat-free diet, plasma was collected at the fasting state (0900) and feeding state (1300). Plasma acyl-ghrelin levels (a), des-acyl ghrelin levels (b), and acyl/total ghrelin ratio (c) were not significantly different between control and fat-free groups. Staining intensities of acyl ghrelin-immunopositive cells from the control and fat-free group were similar (d and e). Ghrelin cell density in the gastric mucosa was not significantly different between the two groups (f). This experiment was replicated twice and the results of 1st data set were used in this figure. MU, mucosal layer; SL, smooth muscle layer; LU, lumen. Scale bars = 25 µm (d and e). Each value represents the mean ± SEM. n = 4.