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. 2018 Jun 15;8:9176. doi: 10.1038/s41598-018-27458-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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mRNA expression analysis by RT-PCR for genes involved in fatty acid synthesis and metabolism in PG-1 cells. Total RNA was extracted from PG-1 cells, cDNA was synthesized, then PCR was performed. PG-1 cells expressed acetyl-CoA carboxylase (Acaca) and fatty acid synthase (Fasn) from the fatty acid synthesis pathway. The mRNA expression of carnitine octanoyltransferase (Crot), CD36 antigen (Cd36), fatty acid binding protein 5 (Fabp5), and acyl-CoA thioesterase (Acot) 1, Acot2, Acot4, Acot6, Acot7, Acot8, Acot9, Acot10, Acot11, Acot12, and Acot13 were detected in PG-1 cells. PC, positive control: adipose tissue (β-actin, Acaca, Fasn, Crot, Cd36, and Acot1-Acot11) or liver (Fabp5, Acot12, and Acot13); NC, negative control (DW).