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. 2018 Jun 15;8:9192. doi: 10.1038/s41598-018-25886-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Structural analyses of frog erythrocytes using 3D RI maps. (a) Cross sections of the RI map of a frog erythrocyte. The red- and blue-dashed regions correspond to the cytoplasm and nucleus, respectively. (b) Bright-field image and DAPI fluorescence image for cross-validation of the cytoplasm and nucleus. (c) 3D-rendered isosurface image of the RI tomogram. (d) RI histograms for the cytoplasm and nucleus in (c).