Fig. 9.

VRK1 contributes to the stability of AURKB. a 293T cells were transfected first with SiControl or SiVRK1-02 and 24 h later transfected using PEI with AURKB (pcDNA3.1/nV5–DEST–AURKB) in combination with V5 and with active HA-ubiquitin (pSSK–HA––Ubiquitin). The immunoprecipitated proteins were determined in immunoblots. b The 293T cells were transfected with plasmids expressing HA–VRK1 (pCEFL–HA–VRK1) and V5-tagged AURKB. 72 h after transfection and overexpression, the 293T cells were treated with 50 µg/mL of cycloheximide and aliquots were taken at different time points during 24 h for protein level determinations. The two experiments were performed three times and the error bars represent the standard deviations. The dashed line represents the 50% level