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. 2018 Jun 15;8:9173. doi: 10.1038/s41598-018-27217-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HSYA preconditioning ameliorates tubular apoptosis induced by I/R. (A) Representative images (magnification, x400) of renal TUNEL assay from rats subjected to sham group, I/R + Vehicle group, and I/R + HSYA. (B) The percentage of TUNEL-positive cells in kidney sections increased following I/R and decreased following treatment with HSYA (*P < 0.05 vs. Sham; ^#P < 0.05 vs. I/R + Vehicle. n = 8 rats per group). (C) Anti-apoptotic effects on HK-2 cell following H/R injury were confirmed by Hoechst 33258 staining. Quantitative analysis of apoptosis is shown in (D). Data were presented as the means ± SD and were representative of three independent experiments; *P < 0.01 versus Control; ^#P < 0.01 versus H/R.