Table 3.
Kinetics of Leptospira infection | Serovar | Species | |
---|---|---|---|
Profiles | Conventional nested PCR (N = 46) | MAT | qRT-PCR-HRM |
C | 1 | Positive (co-agglutination – highest titre 1:1280 against Arb A and Cyn) | L. borgpetersenii |
1 | Positive (Arb A 1:160) | L. borgpetersenii | |
1 | Positive (co-agglutination – highest titre 1:320 against Arb A 1:320) | L. interrogans | |
1 | NC | L. borgpetersenii | |
6 | Negative | L. interrogans | |
B | 1 | NC | L. interrogans |
1 | NC | L. interrogans | |
16 | Negative | L. interrogans | |
10 | Negative | L. borgpetersenii | |
A | 3 | Negative | L. interrogans |
5 | Negative | L. borgpetersenii |
Abbreviations: Arb A [serovar Arborea (Azorean isolate) serogroup Ballum]; Cyn [Cynopteri (reference serovar) serogroup Cynopteri]; NC, not conclusive (specific reactivity below the cut-off of 1:160 adopted in the Azorean endemic region).