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. 2018 Jul;24(7):915–925. doi: 10.1261/rna.064816.117

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© 2018 Negro et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 4. — H₂O₂ triggers polarization of primary SC cultured in microfluidic devices. (A) Scheme of microfluidic devices used in the study. (B) SC (S100 positive, white) plated in the somatic chamber are exposed to H₂O₂ added to the distal one (50 µM for 1 h, right panel). SC processes orientate toward the opposite compartment. Scale bars, 50 µm. (C,D) Orientation score determination. (***) P < 0.001. (E) Anxa2 (green) accumulates close to the grooves' entrance upon H₂O₂ stimulation. SC are identified by S100 staining (white); phalloidin is in red. Scale bars, 20 µm.