Skip to main content
. 2018 May 1;32(9-10):658–669. doi: 10.1101/gad.313460.118

Figure 2.

Figure 2.

Oxidative stress-dependent telomere shortening in MTH1 knockout and MTH1/PRDX1 double-knockout cells. (A–C) TRF length determination by Southern blot analysis of genomic DNA. Genomic DNAs from HCT116 wild-type and knockout cells grown at 20% O2 (A), 40% O2 (B), or 5% O2 (C) for the indicated PDs were purified, restriction-digested, resolved on 0.7% agarose gels, and analyzed upon in-gel hybridization using a random radiolabeled telomeric probe. Averaged rates of telomere shortening (base pairs/PD), indicated below the gels, were deduced by quantifying the changes in telomere length between the indicated consecutive PDs. (D) Comparison of telomere shortening rates between MTH1 knockout (M KO) and PRDX1/MTH1 double-knockout (PM DKO) cells exposed to different O2 concentrations. The graph represents the median TRF lengths as a function of PDs. The graphs for MTH1 knockout (5% O2), MTH1 knockout (20% O2), and PRDX1/MTH1 double knockout (20% O2) were derived from Supplemental Figure S2B, while MTH1 knockout (40% O2) data points were derived from B.