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Reporter gene tests of genomic fragments bound only by Otd. (A) OtdEL3 is bound by Otd at S5 and S6–S8, but not by Bcd, and is inactive. (B) The OtdL2 enhancer is bound by Otd at S6–S8 and is active in the head. (C) Otd-preferred sequence TTATCCT that is enriched in the Otd S6–S8 ChIP data is preferred in vitro by Otd. (D,E) Mutating the Otd suboptimal site TTATCCT abolishes enhancer activity in a late acting Otd enhancer.
