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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Lung Cancer. 2018 May 1;121:82–90. doi: 10.1016/j.lungcan.2018.04.024

Fig. 3. c-Jun expression is induced under hypoxia and upregulated by HIF-1, and c-Jun and HIF-1α contribute to resistance to gefitinib in NSCLC cells.

Fig. 3

A. HCC827, H3255, H1650, and H1975 cells were cultured in normoxia or in a hypoxia chamber (1% O2) for 24 hours. Cells were then harvested immediately, and equal amounts of protein lysates were subjected to Western blot analysis with the indicated antibodies. B. HCC827 and H3255 cells were transfected with a construct containing HIF-1α/ΔODD mutant or pcDNA3.1 control vector as described in Materials and Methods. Cell lysates were prepared for Western blotting with the indicated antibodies. C. HCC827 and H3255 cells expressing control vector or HIF-1α/ΔODD mutant were treated with 20 nM gefitinib or vehicle control for 24 hours. Cell lysates were prepared for Western blotting with the indicated antibodies. The level of β-actin was used as a reference of lysate protein loading control of each cell line.