Table 2. Protein identification by MALDI-TOF/TOF of qualitative changes in the protein abundances of Streptococcus pneumoniae TIGR4 after deferoxamine treatment.
| Spot | Abundance | Locus | Protein name | Scorea | Locationb |
|---|---|---|---|---|---|
| TE1 | Decreased | SP_1284 | LemA protein | 300 | Membrane |
| TE2 | Decreased | SP_1271 | 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase | 349 | Cytoplasmic |
| TE3 | Increased | SP_0845 | Basic membrane family protein | 942 | Extracellular |
| TE4 | Increased | SP_1362 | Glutamate dehydrogenase | 837 | Cytoplasmic |
| TS1 | Decreased | SP_1004 | Uncharacterized protein | 410 | Extracellular |
| TS2 | Decreased | SP_0341 | UPF0371 protein SP_0341 | 877 | Cytoplasmic |
| TS3 | Decreased | SP_0012 | Hypoxanthine-guanine phosphoribosyltransferase | 749 | Cytoplasmic |
| TS4 | Decreased | SP_1229 | Formate–tetrahydrofolate ligase | 650 | Cytoplasmic |
| TS5 | Decreased | SP_0027 | Ribose-phosphate pyrophosphokinase 1 | 665 | Cytoplasmic |
| TS6 | Decreased | SP_0746 | ATP-dependent Clp protease proteolytic subunit | 81 | Cytoplasmic |
| TS7 | Decreased | SP_2190 | Choline binding protein A | 174 | Extracellular |
| TS8 | Decreased | SP_0715 | Lactate oxidase | 686 | Cytoplasmic |
| TS9 | Decreased | SP_2216 | Secreted 45 kd protein | 512 | Extracellular |
| TS10 | Decreased | SP_1456 | Peptide deformylase (PDF) | 251 | Cytoplasmic |
| TS11 | Decreased | SP_0459 | Formate acetyltransferase | 152 | Cytoplasmic |
| TS12 | Decreased | SP_1534 | Probable manganese-dependent inorganic pyrophosphatase | 533 | Cytoplasmic |
| TS13 | Decreased | SP_0516 | Protein GrpE (HSP-70 cofactor) | 178 | Cytoplasmic |
| TS14 | Decreased | SP_2012 | Glyceraldehyde-3-phosphate dehydrogenase | 451 | Cytoplasmic |
| TS15 | Decreased | SP_1445 | GMP synthase [glutamine-hydrolyzing] | 676 | Cytoplasmic |
| TS16 | Decreased | SP_0236 | DNA-directed RNA polymerase subunit alpha (RNAP subunit alpha) | 152 | Cytoplasmic |
| TS17 | Decreased | SP_1922 | Probable transcriptional regulatory protein SP_1922 | 418 | Cytoplasmic |
| TS18 | Decreased | SP_0435 | Elongation factor P (EF-P) | 290 | Cytoplasmic |
| TS19 | Decreased | SP_2084 | Phosphate-binding protein PstS 2 (PBP 2) | 862 | Extracellular |
| TS20 | Decreased | SP_1999 | Catabolite control protein A | 937 | Cytoplasmic |
| TS21 | Decreased | SP_1508 | ATP synthase subunit beta | 261 | Cytoplasmic |
| TS22 | Decreased | SP_0745 | Uracil phosphoribosyltransferase | 702 | Cytoplasmic |
| TS23 | Increased | SP_0862 | Ribosomal protein S3 | 424 | Cytoplasmic |
Notes.
a
Mascot scores >70 were considered significant at p < 0.05.
b
Protein localization in subcellular compartments was assigned using LocateP.