Table 3. Protein identification by MALDI-TOF/TOF of quantitative changes (>2 fold change (FC)) in the protein abundances of Streptococcus pneumoniae TIGR4 after deferoxamine treatment.
| Spot | Abundance | FCa | p-value | Locus | Protein name | Scoreb | Locationc |
|---|---|---|---|---|---|---|---|
| TE5 | Increased | 6.1 | 0.03 | SP_1220 | L-lactate dehydrogenase | 607 | Cytoplasmic |
| TE6 | Increased | 6.9 | 0.01 | SP_0421 | 3-oxoacyl-[acyl-carrier protein] reductase | 528 | Cytoplasmic |
| TE7 | Decreased | 4.6 | 0.04 | SP_2055 | Alcohol dehydrogenase, zinc-containing | 535 | Cytoplasmic |
| TE8 | Decreased | 4.3 | 0.03 | SP_2012 | Glyceraldehyde-3-phosphate dehydrogenase | 255 | Cytoplasmic |
| TS24 | Increased | 4.4 | 0.02 | nanA | Sialidase A | 185 | Cell wall |
| TS25 | Decreased | 4.2 | 0.02 | SP_0499 | phosphoglycerate kinase | 991 | Cytoplasmic |
| TS26 | Decreased | 4.5 | 0.04 | SP_1489 | Elongation factor Tu | 1,120 | Cytoplasmic |
| TS27 | Decreased | 6.6 | 0.03 | SP_0897 | Pyruvate kinase | 708 | Cytoplasmic |
| TS28 | Decreased | 8.8 | 0.01 | SP_2056 | N-acetylglucosamine-6-phosphate deacetylase | 587 | Cytoplasmic |
| TS29 | Decreased | 4.2 | 0.03 | SP_1128 | Enolase | 349 | Cytoplasmic |
| TS30 | Decreased | 4.1 | 0.04 | SP_1541 | 30S ribosomal protein S6 | 114 | Cytoplasmic |
| TS31 | Decreased | 4.4 | 0.04 | SP_1910 | Uncharacterized protein | 523 | Cytoplasmic |
Notes.
a
For increased proteins, the FC was calculated as the treatment/control ratio; for decreased proteins, the FC was calculated as the control/treatment ratio.
b
Mascot scores >70 were considered significant at p < 0.05.
c
Protein localization in subcellular compartments was assigned using LocateP.