Table 1. Chemical properties of substrate.

Mean ± standard deviation. Statistical analysis was carried out by ANOVA using SPSS 19.0 software. The Post Hoc tests were done with LSD method. Different lower-case letters showed significant difference (P < 0.05) in the substrate chemical properties between the different growth stages of G. lucidum.

Growth stage	pH	Moisture (%)	Total nitrogen (g/kg)	Total phosphorus (g/kg)	Total potassium (g/kg)
Hyphal stage	4.34 ± 0.11 c	64.60 ± 1.08 a	13.53 ± 0.30 a	2.74 ± 0.13 b	13.31 ± 0.34 b
Budding stage	5.29 ± 0.15 a	58.01 ± 1.22 b	10.75 ± 0.29 b	3.36 ± 0.08 a	15.45 ± 0.13 a
Elongation stage	5.05 ± 0.01 b	54.48 ± 2.02 c	11.25 ± 0.59 b	1.64 ± 0.07 d	8.80 ± 0.19 d
Mature stage	4.89 ± 0.06 b	38.56 ± 1.54 d	12.60 ± 0.04 a	2.43 ± 0.03 c	11.47 ± 0.68 c