Table 2. Sites diversity in sequence reads predicted by sites diversity in references and by GC content.
The results are reported for sequences assigned to Coleoptera order over the 5′ and 3′ end of coxI barcode region.
| 5′coxI barcode analyzed sites: 1-400 | 3′coxI barcode analyzed sites: 200-end | |||||
|---|---|---|---|---|---|---|
| GCref | Dref | Residuals | GCref | Dref | Residuals | |
| Degree of freedom | 1 | 1 | 562 | 1 | 1 | 562 |
| Sum of squares | 4.518 | 58.766 | 123.808 | 37.999 | 50.025 | 135.407 |
| Mean square | 4.518 | 58.766 | 0.220 | 37.999 | 50.025 | 0.241 |
| F-value | 20.507 | 266.754 | – | 157.71 | 207.63 | – |
| Explained variance (%) | 2,41 | 31,41 | 66,17 | 17 | 22,3 | 66,17 |
| Pr(>F) | 7.257e−06*** | <2.2e−16*** | – | <2.2e−16*** | <2.2e−16*** | – |
Notes.
GCref and Dref are the GC content and sites diversity of reference sequences respectively.
Significance codes
- ***
- 0
- **
- 0.001
- *
- 0.01
- .
- 0.05