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. 2018 Jun 13;6:e4980. doi: 10.7717/peerj.4980

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©2018 Carew et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Individual non-destructive DNA extraction (Extraction 1) followed by a Chelex extraction (Extraction 2) performed on single specimens from 15 different macroinvertebrate orders (Component 1). (B) Comparison of the detection of taxa via metabarcoding by using three DNA extraction based on non-destructive processing, homogenization of whole small taxa and dissection of all taxa (Component 2). (C) Comparison of the detection of taxa via metabarcoding from six field collected samples stored in different ethanol concentration, and temperatures an with varying levels of diversity. Drawings by Melissa Carew.