Superoxide anion and hydrogen peroxide were measured by lucigenin-derived chemiluminescence (A) and amplex red (B) in VSMCs stimulated with angiotensin II (Ang II, 100nmol/L) for 5 minutes in the presence of NoxA1ds (N1) (10µmol/L, Nox1 inhibitor), NoxA1ds scrambled control (Scb) or GKT137831 (GKT) (10µmol/L, Nox1/4 inhibitor). Irreversible protein oxidation was assessed by western blot of PTP oxidation (C) and peroxiredoxin hyperoxidation (D) in the presence of Nox inhibitors. Results are expressed as mean±SEM of 6-10 separate experiments.*p<0.05 vs WKY control (Ctl), +p<0.05 and ++p<0.01 vs respective Ctl, #p<0.05 vs SHR Ctl and &p<0.05 vs SHR N1.