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. Author manuscript; available in PMC: 2019 Jun 15.
Published in final edited form as: Clin Cancer Res. 2018 Mar 16;24(12):2780–2793. doi: 10.1158/1078-0432.CCR-17-3112

Figure 3.

Figure 3

Activation of TGF-β signaling by chemotherapeutic agents in human cervical cancer cells and ovarian cancer cells. Cervical cancer cell lines HeLa (A) and C-4I cell (B), and ovarian cancer cell lines OVCAR-3 (C) and TOV-21G (D) were treated with 10μM cisplatin (DDP), 10nM paclitaxel (PTX), 100nMdoxorubicin (Doxo) or 250nMcamptothecin(CPT) for 1h, 6h or 24h. The cells were harvested and their extracts were used for western immunoblotting for the detection of phosphorylated Smad2 (P-Smad2) and of phosphorylated Smad3 (P-Smad3). Total Smad2 (T-Smad2) and total Smad3 (T-Smad3) were used to verify equal sample loading.