Figure 1. TMT reagents and peptides used in this experiment.
A) The table lists the phos9 phosphopeptide sequences, m/z for TMTzero- and TMTsh-labeled phosphopeptides, isoelectric point for unlabeled phosphopeptide, and associated protein. B) Chemical structures of TMTzero and TMTsh. C) Spectrum highlighting the mass difference between a doubly charged, lysine-terminating peptide (DLPEPIpSPETK) labeled with TMTzero and TMTsh.