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. Author manuscript; available in PMC: 2019 Jun 15.
Published in final edited form as: Cancer Res. 2018 Apr 18;78(12):3207–3219. doi: 10.1158/0008-5472.CAN-18-0222

Figure 6. p21 plays an oncogenic role in promoting tumor cell proliferation and anti-apoptosis.

Figure 6

(A) Cell proliferation decreased after p21 siRNAs treatment in PC-9, H1975, H2228, H1299 and H838 cell lines at 96 h – 120 h measured by WST-1 assay. (B and C) Changes in cell proliferation after MIR22HG, p21 or both siRNAs treatment in PC-9 and H838 at 96 h measured by WST-1 assay. (D) The protein expression of cleaved PARP after MIR22HG, p21 or both siRNAs treatment in PC-9, H1975 and H838 cells measured by western blot. (E and F) CCNE1 protein expression after p21 siRNA (E) and MIR22HG siRNA (F) treatment at 72 h in lung cancer cells. (G) Western blot showing the total MET and p21 proteins expression after transfection of MIR22HG over expression construct or both siRNAs of MET and MIR22HG. (H) Quantitative value of image G by Image J.