. 2017 Nov 21;6(4):197–203. doi: 10.22088/BUMS.6.4.197

Table 2.

The primer sequences of RFLP, sequencing, and PCR conditions for the studied SNPs

SNP	Type of primers	Primer sequences 5’ 3’	Enzyme	PCR condition ( ^o C/s )			DNA/ fragment size (bp)
SNP	Type of primers	Primer sequences 5’ 3’	Enzyme	Den.	Ann.	Ext.	DNA/ fragment size (bp)
rs1801214	PCR-RFLP	F:TTAGCCACCTGGTCGTCGTCAA	Hind II	95/30	64/30	72/30	123/106+17
	PCR-RFLP	R:AGGGCACAAGGTAGCAGTAGGTGC		95/30	64/30	72/30	123/106+17
	Sequencing	F:GCAACCTCACCATCGACTTC		95/30	59/30	72/30	600
	Sequencing	R:AGGGCACAAGGTAGCAGTAG		95/30	59/30	72/30	600
rs1046320	PCR-RFLP	F:CTTTCACCAGTGCCGCCTGTG	Sph I	95/30	60/30	72/30	96/77+19
	PCR-RFLP	R:GTATTCCCTTTGTCGGGGTGCA		95/30	60/30	72/30	96/77+19
	Sequencing	F:GATCGAGTTCAGCACCATCCT		95/30	60/30	72/30	679
	Sequencing	R:AAAGGGGAAGAGCTGCTAAGG		95/30	60/30	72/30	679

Den: denaturation; Ann: annealing; Ext: extension.