Figure 2.
Histone variant chaperones and pre-nucleosomal posttranslational modifications of CENP-A. (a) The interaction of histone H3 variants with distinct chaperone proteins determines the timing and site of variant nucleosome deposition. (b) The amino-terminus of prenucleosomal CENP-A is trimethylated and phosphorylated at Ser16/18. Recruitment of the HAT1 acetyltransferase through RbAp46/48 binding to CENP-A and histone H4 leads to acetylation of the histone H4 tail within the prenucleosomal complex.