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. Author manuscript; available in PMC: 2018 Jun 17.
Published in final edited form as: Curr Opin Cell Biol. 2018 Apr 2;52:126–135. doi: 10.1016/j.ceb.2018.03.003

Figure 3.

Figure 3

Cell cycle control of vertebrate CENP-A deposition. (a) CDK1 activity increases in G2/M phase and inhibits the deposition of CENP-A nucleosomes by directly phosphorylating Mis18BP1 and the CENP-A chaperone HJURP. Mis18BP1 phosphorylation disrupts the binding to the Mis18α/β oligomer and thus inhibits recruitment of the complex to centromeres. Reduction of CDK1 activity in response to activation of the APC/C following mitotic checkpoint satisfaction leads to CENP-A deposition in G1. PLK1 activity positively regulates CENP-A deposition through phosphorylation of Mis18BP1. FACT mediated transcription in Drosophila and INO80 remodeling complex in budding yeast promote histone exchange within centromeric chromatin (reviewed by [70]). (b) CDK1 targeted phosphorylation sites within Mis18BP1 and HJURP that are known to inhibit CENP-A deposition are depicted.