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. Author manuscript; available in PMC: 2018 Nov 27.
Published in final edited form as: Genet Med. 2018 Mar 8;20(10):1224–1235. doi: 10.1038/gim.2017.251

Figure 5. Administration of 2-oxoadipate and quinolinic acid in vitro was more toxic for neuronal cells than fibroblasts.

Figure 5

(a) Western blot analysis detected normal expression in control fibroblasts after supplementation of 50 μM quinolinic acid and 5 μM 2-oxoadipate after 4 days. (b) Quantification of protein levels were normalised to porin. (c) In human neuroblastoma cells (SHSY-5Y) the supplementation of the 50 μM quinolinic acid and 5 μM 2-oxoadipate resulted in significantly reduced levels affecting all five OXPHOS complexes, as shown in (d) (n=3 experimental replicates). P values are from One-Way ANOVA test. Where indicated * P<0.05. (e) Visualization of apoptotic cells stained with annexin V-FITC using confocal microscopy after treatment with only 50 μM quinolinic acid and combination of 50 μM quinolinic acid and 5 μM 2-oxoadipate. Cells were supplemented for 4 days before staining. DAPI staining was used as nuclear marker. Top row shows fibroblasts where no Annexin V positive cells were detected in any treatment. Bottom row shows SHSY-5Y cells: Indication annexin V positive cells were identified after double supplementation. Scale bar = 50μM.