Figure 2.

Characterization and optimization of stable HTC/AvBD9-luc luciferase reporter cells for high-throughput screening. (A) The fold change in luciferase activity of each cell clone in response to 8 mM butyrate relative to that of non-stimulation control. (B) Dose-dependent response of two selected stable cell clones and stable cell mixture to sodium butyrate (mM). The results in (A,B) are means ± SEM of two independent experiments. (C) Relative luciferase activities of stable reporter cells in the presence or absence of 8 mM sodium butyrate for calculation of the Z'-factor.