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. 2018 Jun 11;9:1331. doi: 10.3389/fimmu.2018.01331

Figure 7.

Figure 7

mmu-miR-21a-5p modulates GPB5 expression and Brucella abortus infection in macrophages. Bone marrow-derived macrophages (BMDMs) transfected with mimic or inhibitor for mmu-miR-21a-5p were infected with B. abortus and the level of guanylate-binding protein (GBP)5 mRNA was measured by real-time PCR (A). The results were normalized to β-actin. Error bars represent the mean ± SD. Similar results were obtained in two independent experiments. Statistically significant differences of GBP5 expression from infected cells to non-transfected (NT)/non-infected (NI) cells are denoted by an asterisk (p < 0.05), and # (p < 0.05) represents statistically significant differences compared with NT/infected (S2308) cells. (B) Expression of GBP5 protein in cytosol from NI and B. abortus-infected BMDMs was assessed by immunoblotting assay. BMDMs were transfected with mimic (MIMIC21) or inhibitors (INH21) for mmu-miR-21a-5p in C57BL/6 cells. The symbol + indicates cells infected with B. abortus S2308 and − indicates NI cells. β-actin was used as a loading control. Values below each band indicate the quantification of band intensity relative to the loading control. Data are representative of two independent experiments. (C) Intracellular growth of B. abortus in macrophages transfected with mimics or inhibitor was assessed by CFU analysis at 24 h post-infection. Error bars represent the mean ± SD. Statistically significant differences compared with NT/S2308 cells are denoted by an asterisk (p < 0.05), and & (p < 0.05) represents statistically significant differences compared with scramble controls.