Table 2.
Macro- and micromorphological features of plant endosphere- and root zone soil-associated black aspergilli isolated from symptomatic sisal plants in the study areas.
| # | Code | Colony morphology (CYA for 7 days at 25°C) | Colony growth at 7 days (mm) 25°, 30°C and 37°C | Conidial head shape | Conidiophore | Vesicle size (μm) | Metulae size (μm) | Phialides | Conidia | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CYA | MEA | OA | Stipe description | Size (μm) | Shape | Size (μm) | Diameter (μm) | Shape and ornamentation | ||||||
| 1 | RSDI | Black colonies with a white leading edge | 40–70 | 45–70 | 40–60 | Radiated | Biseriate, smooth, and colorless | 224–787 × 10–16.5 | 22.5–65 | 6.5–16 × 3 | Ampuliform | 4.7– 10.7 × 3 | 3–4 | Light brown to dark brown, globular, rough to finely rough |
| 2 | RSDII | Black colonies with a white to yellow leading edge | 16–60 | 20–70 | 29–65 | Radiated | Biseriate, smooth, and colorless | 800–900 × 12.5 | 40–65 | 12.5 × 3–5 | Ampuliform | 7.5 × 3 | 2.5–5 | Dark brown to black, globular, smooth to finely rough |
| 3 | RRI | Black colonies with a white to yellow leading edge | 40–70 | 45–70 | 45–70 | Radiated | Biseriate, smooth, and colorless | 900–977 × 4.5–17 | 51–68 | 10.5–24 × 3 | Ampuliform | 9–13 × 3 | 2.5–4 | Dark brown to black, globular, smooth to finely rough |
| 4 | RRII | Black colonies with a white to yellow leading edge | 35–60 | 25–66 | 46–70 | Radiated | Biseriate, smooth, and colorless | 750–900 × 15 | 40–50 | 10–12.5 × 3–5 | Ampuliform | 7.5 × 3–5 | 3–5 | Dark brown to black, globular, rough to finely rough |
| 5 | RCI | Black colonies with a white to yellow leading edge | 22–58 | 56–60 | 50–72 | Radiated | Biseriate, smooth, and colorless | 500–900 × 15–12.5 | 30–65 | 10–12.5 × 5 | Ampuliform | 5 × 3 | 3–5 | Dark brown to black, globular, rough |
| 6 | RCII | Black colonies with a white to yellow leading edge | 20–48 | 20–65 | 35–60 | Radiated | Biseriate, smooth, and colorless | 600–900 × 12.5–15 | 40–65 | 12.5–15 × 3–5 | Ampuliform | 5–7.5 × 3 | 2.5–5 | Dark brown to black, globular, rough to finely rough |
| 7 | CSDI | Black colonies with a white leading edge | 40–70 | 45–70 | 45–70 | Radiated | Biseriate, smooth, and colorless | 400–600 × 15–16 | 49–61 | 12–20 × 3 | Ampuliform | 10–12 × 3 | 3–4.5 | Dark brown to black, globular, rough to finely rough |
| 8 | CSDII | Black colonies with a white to yellow leading edge | 40–70 | 45–70 | 45–70 | Radiated | Biseriate, smooth, and colorless | 238–584 × 8–16 | 20–60 | 6–12.5 × 3 | Ampuliform | 8–10 × 3–4 | 3–5 | Dark brown to black, globular, rough to finely rough |
| 9 | CRI | Black colonies with a white leading edge | 40–70 | 35–70 | 35–60 | Radiated | Biseriate, smooth, and colorless | 400–900 × 10 | 40–60 | 15–20 × 3 | Ampuliform | 7–10 × 3 | 2.5–4 | Dark brown to black, globular, rough |
| 10 | CRII | Black colonies with a white to yellow leading edge | 40–70 | 35–70 | 45–65 | Radiated | Biseriate, smooth, and colorless | 250–1000 × 10 | 30–55 | 6.5–11 × 3 | Ampuliform | 6–10 × 3 | 3–5 | Brown to black, globular, finely rough |
| 11 | CCI | Black colonies with a white leading edge | 40–70 | 45–70 | 45–70 | Radiated | Biseriate, smooth, and colorless | 620–900 × 10 | 40 | 7–16 × 3 | Ampuliform | 7.5–14 × 3 | 2–3.5 | Light brown to dark brown, globular, rough to finely rough |
| 12 | CCII | Black colonies with a white leading edge | 15–70 | 35–70 | 20–65 | Radiated | Biseriate, smooth, and colorless | 200–550 × 10 | 22 | 7–18 × 6–10 | Ampuliform | 8.5–12 × 5–6 | 5 | Brown to black, globular, rough to echinulate |
| 13 | FSDI | Black colonies with a white to yellow leading edge | 18–60 | 22–55 | 45–60 | Radiated | Biseriate/smooth, and colorless | 400–900 × 7.5–10 | 20–45 | 13–15 × 3–5 | Ampuliform | 5–7 × 3–4 | 3–5 | Dark brown to black, globular, rough to finely rough |
| 14 | FSDII | Black colonies with a white to yellow leading edge | 18–64 | 22–60 | 45–60 | Radiated | Biseriate/smooth and colorless | 400–900 × 7.5–10 | 20–50 | 12.5–15 × 3–5 | Ampuliform | 5–10 × 3 | 2.5–5 | Dark brown to black, globular, rough to finely rough |
| 15 | FRI | Black colonies with a white to yellow leading edge | 40–70 | 35–90 | 35–70 | Radiated | Biseriate (uniseriate rare)/smooth and colorless | 382–772 × 9–15.5 | 34–64.5 | 10–20 × 3 | Ampuliform | 7–9 × 3 | 2.5–4 | Dark brown, globular, rough to finely rough |
| 16 | FRII | Black colonies with a white to yellow leading edge | 22–57 | 20–55 | 30–52 | Radiated | Biseriate/smooth and colorless | 400–640 × 10–12.5 | 22.5–52.5 | 14 × 3–5 | Ampuliform | 7 × 3–4 | 3–4 | Dark brown to black, globular, rough |
| 17 | FCI | Black colonies with a white to yellow leading edge | 45–90 | 35–80 | 35–80 | Radiated | Biseriate (uniseriate rare)/smooth and colorless | 285–500 × 12–14.5 | 32.5–60.5 | 15–20 × 3 | Ampuliform | 8–11 × 3 | 2.5–3.5 | Dark brown to black, globular, rough to finely rough |
| 18 | FCII | Black colonies with a white to yellow leading edge | 18–60 | 24–70 | 38–65 | Radiated | Biseriate/smooth and colorless | 650–1050 × 10–12.5 | 30–52.5 | 10–12.5 × 3 | Ampuliform | 7.5 × 3 | 2.5–3 | Dark brown to black, globular, rough to finely rough |
| 19 | SSDI | Black colonies with a white leading edge | 40–70 | 35–70 | 38–70 | Radiated | Biseriate (uniseriate rare)/smooth and colorless | 577–726 × 10–13 | 27–61 | 11–12 × 3 | Ampuliform | 5.8–10.5 × 3 | 2.5–4 | Brown to black, globular, rough to finely rough |
| 20 | SSDII | Black colonies with a white to yellow leading edge | 25–40 | 40–50 | 38–60 | Radiated | Biseriate/smooth and colorless | 380–660 × 10–12.5 | 27.5–45 | 9–10 × 4–5 | Ampuliform | 6 × 4–5 | 3–4 | Dark brown to black, globular, finely rough |
| 21 | SRI | Black colonies with a white leading edge | 27–65 | 41–52 | 29–70 | Radiated | Biseriate/smooth and colorless | 600–1000 × 10–12.5 | 35–57.5 | 7.5–12,5 × 3 | Ampuliform | 5–10 × 3 | 2.5–3 | Dark brown to black, globular, smooth to finely rough |
| 22 | SRII | Black colonies with a white to yellow leading edge | 16–60 | 20–70 | 29–65 | Radiated | Biseriate/smooth and colorless | 800–900 × 12.5 | 40–65 | 12.5 × 3–5 | Ampuliform | 7.5 × 3 | 2.5–5 | Dark brown to black, globular, smooth to finely rough |
| 23 | SCI | Black colonies with a white to yellow leading edge | 40–70 | 35–70 | 38–70 | Radiated | Biseriate/smooth and colorless | 400–900 × 7.5–10 | 20–45 | 13–15 × 3–5 | Ampuliform | 5–7 × 3–4 | 3–5 | Light brown to dark brown, globular, rough to finely rough |
| 24 | SCII | Black colonies with a white to yellow leading edge | 35–60 | 25–66 | 46–70 | Radiated | Biseriate/smooth and colorless | 400–600 × 15–16 | 49–61 | 12–20 × 3 | Ampuliform | 10–12 × 3 | 3–4.5 | Light brown to black, globular, rough to finely rough |