Table 1.
Biological pathways enriched by DAVID in STIM1 KD hNPCs.
| Go term | Pathway | P -value | Fold enrichment | Bonferroni | Benjamini | FDR | Genes |
|---|---|---|---|---|---|---|---|
| Upregulated | |||||||
| GO:0007268 | Chemical synaptic transmission | 6.96E-05 | 6.491 | 0.038 | 0.019 | 0.101 | NRXN2, KIF5A, NPTX2, GRIK4, DLG4, CHRNA4, PRKCG, CACNB3, CACNA1B |
| GO:0007158 | Neuron cell-cell adhesion | 0.003 | 32.458 | 0.876 | 0.407 | 5.265 | NRXN2, NLGN4X, ASTN1 |
| GO:0051899 | Membrane depolarization | 0.008 | 20.773 | 0.993 | 0.637 | 12.289 | CHRNA4, CACNB3, CACNA1B |
| GO:0030534 | Adult behavior | 0.009 | 19.974 | 0.995 | 0.598 | 13.201 | NRXN2, NLGN4X, SHANK1 |
| GO:0007411 | Axon guidance | 0.013 | 5.443 | 0.999 | 0.656 | 17.590 | KIF5A, NGFR, UNC5C, CHL1, SLIT3 |
| GO:0060997 | Dendritic spine morphogenesis | #0.066 | 28.852 | 1.0 | 0.950 | 63.527 | DLG4, SHANK1 |
| GO:0007399 | Nervous system development | #0.082 | 3.015 | 1.0 | 0.933 | 71.593 | IGSF8, CPLX2, DLG4, SPOCK1, ELAVL3 |
| Downregulated | |||||||
| GO:0006364 | rRNA processing | 1.62E-07 | 6.130 | 2.21E-04 | 2.21E-04 | 2.66E-04 | EMG1, PNO1, EXOSC5, RPS27L, DIEXF, MRTO4, NOP14, EBNA1BP2, PA2G4, DKC1, DHX37, DDX21, PES1, LTV1, WDR43 |
| GO:0008283 | Cell proliferation | 1.00E-06 | 4.301 | 0.001 | 6.82E-04 | 0.001 | POLR3G, TP53, CD70, MCM10, PRDX1, CDC25A, PLCE1, PA2G4, DKC1, ASCC3, FRAT2, TXNRD1, NRG1, LRP2, PES1, MYC, EMP1, GNL3 |
| GO:0000082 | G1/S transition of mitotic cell cycle | 2.43E-06 | 8.574 | 0.003 | 0.001 | 0.003 | CCNE1, CDC6, CDC45, CDKN1A, RRM2, ID4, CDK6, RCC1, MCM10, CDC25A |
| GO:0006260 | DNA replication | 4.01E-04 | 5.078 | 0.420 | 0.127 | 0.655 | EXO1, CDC6, CDC45, POLE3, RRM2, MCM10, C10ORF2, CDC25A, DSCC1 |
| GO:0042771 | Intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator | 4.01E-04 | 14.106 | 0.420 | 0.103 | 0.656 | CDKN1A, AEN, TP53, RPS27L, PHLDA3 |
Top biological pathways up- and down-regulated in STIM1 KD NPCs vs. control cells. Fisher Exact P -values are shown and GO terms are arranged according to their FDR value (False Discovery Rates). All over-represented pathways had a fold change > 2. Both Benjamini–Hochberg and Bonferroni multiple testing correction methods for the occurrence of false positive identifications by adjusting p-values are given. Shown are the gene lists identified in our data set and associated with each pathway. # Indicates p -value > 0.05.